lu-b1locilocatedonb-genomewerehigherthanthoseofrelativelociona-andd-genomes.theseresultssuggestedthatthegeneticdiversityatgliwashigherthanthatofglu-1,andthegeneticdiversityofstorageproteingenesonb-genomewashigherthanthatofa-andd-genomes.
7.fiveecialpcrprimersforglu-ax,glu-bx,glu-a3,glu-b3andglu-1dx5,and2rprimersforγ-gliadinandlmw-gluteninwereusedtoinvestigatedthestorageproteingenevariatioamong8sichuanwhitewheatlandraces,14yuanhulledwheatacceio,9tibetanweedraceand9xinjiangricewheatacceio.thepcranalysisindicatedthatlowlevelgeneticdiversitywerefoundatglu-ax,glu-bx,glu-a3andglu-b3,whilehighlevelgeneticdiversitywerefoundedattherlociofγ-gliadinandlmw-glutenin.theecialdnafragmentofglu-1dx5wasnotdetectedamongall40acceio.itindicatedthattheredidnothavesubunit5encodedbyglu-d1intheseacceio.
8.using28primerset-enzymecombinatioof14sts-pcrmarkersand24rmarkers,thegeneticvariatioamongsichuanwhitewheat,yuanhulledwheat,tibetanweedraceandxinjiangricewheatwereinvestigated.elevenoutof14sts-pcrprimersets(78.6)and16outof28primerset-enzymecombinatio(57.1)werepolymorphic,producingatotalof121fragmentswithanaverageof4.3fr
agmentsperprimerset-enzymecombinatio.at24rloci,21rloci(87.5)werepolymorphic,detectingatotalof83alleleswithanaverageof3.5allelesperrloci.
9.thenei'sgeneticsimilarity(gs)indexeswithinandbetweengrouwerecalculatedbasedonthests-pcrandrdata.thegsamongthegrouofsichuanwhitewheatandyuanhulledwheat,revealedbysts-pcrandrmarkers,werehigherthanthatoftibetanweedraceandxinjiangrice
上一页 [1] [2] [3] [4] [5] [6] [7] [8] [9] [10] 下一页