d1b3of1rs.pcranalysisindicatedthatthe10-aalsopoeedthe1rsofrye.usingfluorescencelabeledtotalgenomicdnaofryeasprobesandcommonwheatgenomicdnaofchineseringforblocking,insituhybridizationshowedthatthe1rsofryewastraferredtomultisikeletwheatline10-a.therestrictionfragmentslocatedontheshortarmofchromosome1bweremiingandtherestrictionfragmentsof1rswerepresentwhentheprobes,whichhavebeenidentifiedontheshortarmofthehomologousgroup1,wereusedinrflpanalysis.fishandrflpanalysisindicatedthe10-adidnotpoethe4a-4rwheat-ryetralocationchromosome.theseresultssuggestedthatthemultiikeletwheatline10-acarriedthet1bl.1rswheat-ryetralocationchromosome.inthisstudy,somerecombinantswithmultiikeletderivedfromatriplecro,10-a/88-1643//chuanyu12,werealsodetected.allofthemalsopoethet1bl.1rstralocationchromosome.
2.acomparisonofsomenormalandmolecularmethodsforidentifyingandsurveyingthepresenceoft1bl.1rstralocationinwheatwasconducted.theresultindicatedthatapageistheeasiestandoftenfasterforscreeningpurposesinwheatbreeding.
3.theeffectsoft1bl.1rstralocationchromosomeontheperformanceofagronomiccharacters,thegrainproteincontent,andthesimpleandpartialcorrelationcoefficientsamongcharacterswereinvestigatedwith4rflpmarkersand1gliadinlocusgld1b3inrecombinantsderivedfromatriplecro,10-a/88-1643//chanyu12.thet1bl.1rstralocationchromosomehadsignificenteffectsonikeletnumberperike,graiperikelet,1000-grainweight,graiweightperike,plantheightandgrainproteincontent,whilenosignificenteffectsongraiperikeandheadingdatewasdetected.

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