andparametriclinkageanalyses.
results:twolocionchromosome9d9s171andd9s175showedsuggestiveevidenceforlinkage,withzall-scoreof3.29and2.94reectively,andp-valueof1.19′10-4and4.73′10-4.thecorreondinglod-scoreofthesetwolociwere4.61and4.29reectivelywiththep-valueof2.44′10-5and5.14′10-5,underadditivemodel.alocusonthelongarmofchromosome20d20s196showedariseofzall-score(from1.52to2.92)andacorreondingdecreaseofp-valuefrom0.04to6.53′10-4whenfamilieswithlowerbmiwereanalyzedalone.otherlociwithweakerevidenceforlinkagewerealsooerved.
conclusio:ourresultssuggestthatchromosome9maycontaingenesinvolvedinthesusceptibilitytotype2diabetesineastandsoutheastchinesehanpopulation,whilechromosome20mayhidegenesthatwerelinkedtotype2diabetesinfamilieswithlowerbmi.otherregiomayalsohidesusceptibilitygeneswithminoreffect,butfurtherinvestigationisrequiredtoconfirmtheresults.
partiistudyonthesinglenucleotidepolymorphism()ofcandidategenesneard9s175
aims:tofindoutofcandidategenesneard9s175inchinesepopulationandstudytheirrelatiohipwithtype2diabetes.
methods:bidirectionalsequencingwasusedtodetectinthe5’regulatoryregio,exoandpartialintroclosetoexoofsomecandidategenesneard9s175.polyphredwasusedtodetectinindividualsamples.apreliminaryestimationofthedistributionofallelesindiabeticpopulationandnormalcontrolpopulationwasperformedusingthecomparisonoftheratioofpic-heightofdnapoolofdiabeticpatientsandthatofnormalcontrols.
results:fourgenesneard9s175werescreenedfor,theywererorb,flj20808,flj20087andfrda.9were