imalmodelofrats
twentymalesdratsreceiveddailyipinjectionof25ml4.25glucosedialysissolutionwithout(hp,n=8)orwith0.025hyaluronan(ha,n=6)foroneweek,anothersixratsdidnotreceiveanyperitonealinjection.twenty-fourhoursafterthelastinjection,a4hdwellstudyusing25ml4.25glucosedialysissolutionwithipvolumemarkerandfrequentdialysateandbloodsamplingswasperformedineachrat.
resultipvwassignificantlyhigherinthehagroupascomparedtothehpgroup(anovarepeatedmeasurement,p<0.01).theperitonealfluidaorptionrate,ke,wassignificantlyincreasedinthehpgroupascomparedtotheothergrou.thedirectlymphaticfluidaorptionrate,keb,wassignificantlyhigherinthetwodailyinfusiongrou(hpandha)ascomparedtothecontrolgroup.however,thetiueaorptionrate,ket,wassignificantlyhigherinhpgroupascomparedwithhagroupandcontrolgroup.
2.ultrastructuralidentificationofanovelmembraneonperitonealsurfaceandelectrogoldmicroscopicinvestigation.
normalratperitoneumwastakenfromfoursdrats.thetiueswereimmediatelyfixedusingoneofthefollowingfixativesfor24hoursbeforeconventionaltiueproceing:(1)2.5g

lutaraldehydeand2polyformaldehyde(control);(2)2.5glutaraldehydeand0.5cetylpyridinumchloridetofixmainlytheglycosaminoglycan(gag);(3)2oso4tofixthephoshpolipids(ph1);(4)4oso4(ph2)and(5)3taicacid(taicacidgroup).afterpostfixiation,dehydration,embedmentandultrathinsection,thesectiowereoervedinahitachi-600tramiionelectronmicroscope.twelvenickelgrids(200mesh)weretouchedgentlyontoperitonealsurfaceofratsfortwosecondsandthenfixedasfollowing:formaldeh

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