erecd4＋tcell-dependent.theresultsofanalyzingtcellreoestomaleproteinordafterrbcg·maleimmunizationshowedthatinitialth1reoestobcgorrbcgweremodulatedduringthecourseofinfectiontobecomeamixedth1/th2reoes.toverifythisphenomenonofth1/th2shift,wefurtheranalyzedthekineticreoestomalepeptidesafterrbcg·maleimmunization.tcellreoesagaitp68-82andp277-291wereshiftfromth1typetoth2type,thenbecomeamixedth1/th2reoe.intriguingly,tcellreoestop100-114weretypicalshiftofth1/th2reoes,whileonlyth2reoestop151-165weredetectedatlatertimepoints.thisrevealedthatmaleproteinhadfunctionaldiversityoftcellepitopes.alltheresultsinthisstudysuggestedthatthecharacteristicsofcd4＋tcellreoesinducedbyrbcg·malehadinitialtendencytohighlevelofth1reoes,andlateron,thereoesbecametoshiftfromth1toth2andgotmixtureofbothtypesofreoesundertheregulationofth1/th2balancemechanism.thisiscorrelatedwellwiththeabilityofbothmyeloid(cd8α－)andlymphoid(cd8α＋)dcsuetstostimulatemale-ecifictcellsfollowingrbcg·maleinfection.inaddition,preliminaryresultsfrommiceimmunizedwith3differentrbcg·malestraialsosuggestedthatthenatureoftcellreoesagaitmaleproteinoritspeptideswereinfluencedbydifferentlevelsandlocalizationofmaleexpreionaccordingtothedifferentexpreioncaettesforrbcg.theseresultsalsoshowsomenewcluesfortherationaldesignofvaccines.