however,thepresenceofimmunogenicmalepeptides/mhcⅱcomplexeswasdetectedexvivoondc,butnotonm?,usingtcellhybridomasecificforthemaleprotein.likewise,upregulationofcd40,b7.1andb7.2moleculesandproductioofil-12p40followinginfectionwasonlyoervedfordc,confirmingtheexclusiveroleofdcinstimulatingtcellreoes.cd8α＋andcd8α－leendcwereequallypotentantigenpresentingcellsinvivo,buttheproductionofil-12wasmainlyaociatedwiththecd8α＋suet.altogether,thesedataindicatedthatinvivodcplayedaprimaryrolenotonlyinacquiredimmunitytomycobacteriaintheearlytimesofinfection,butalsoiniateimmunitythroughil-12secretion.strikingly,bcgbacillussurvivebutremainstableinnumberinthedcduringtheearlystagesofinfection.asantigenpresentationbydcisrapidlylost,thissuggeststhatdcmayrepresentahiddenreservoirformycobacteria.theseresultswereveryusefulforthepreventionoftuberculosisandrelatedinfectio,andforthedevelopmentofnewvaccinesbasedonbcgvector.
3.kineticsoftcellimmunereoesinducedbyrecombinantmycobacteriumbovisbcgexpreingmaleprotein
inordertodeterminethecharacteristicsoftcellreoesinducedbyrbcg·male,thelenocytesfromimmunizedmiceweretreatedtodepletecd4＋orcd8tcellsbyanimmunomagneticselection,thenthesecellswereusedtomeasuretheifn-γoril-2reoestomaleproteinoritspeptidesineliottest.noifn-γoril-2reoesagaitmaleanditspeptidesweredetectedafterdepletionofcd4＋tcells,incontrast,highlevelofifn-γoril-2reoesagaitmaleanditspeptideswerestilldetectedafterremovingcd8＋tcells.theseresultsclearlyindicatedthattc
ellreoesinducedbyrbcg·malew