lsedwithrbcg·male、bcg·wtorpurifiedmaleprotein.fourtcellepitopesofmaleproteinpresentedonthesurfaceofthosedcspulsedwithrbcg·maleandpurifiedmaleproteinwererecognizedbycd4＋thybridomasecificformalepeptidesp68-82,p100-114,p151-165andp277-291,reectively,whereasdcspulsedwithbcg·wtfailedtostimulatethesethybridomas.theresultsalsoshowedthatrbcg·maleinducedinvivotcellproliferationandifn-γreoesagaitmaleproteinanditspeptidesordantigenwhilebcg·wtonlytriggeredreoesecificford.rbcg·malefunctionallyexpreedthefourtcellepitopesofmaleproteinandepitopemaingfrommiceimmunizedwithrbcg·maleshowedthatnonovelepitopesorcrypticepitopeswererevealedinrbcg,andthep68-82wasimmunodominantepitopeandtheothersweresubdominantepitopes.itwasfurthershownthatbcgisoneofthemostpromisinglivevectorstoexpreanddeliverforeignantigetotheimmunesystem.
2.theroleofdendriticcellsduringtheearlystagesofarecombinantmycobacteriumbovisbcginfection
phagocyticcellswithapcfunctioplayanimportantr
oleinresistancetobacterialinfectioninturnthattheycanstimulatetcellreoesenhancingbacterialclearance.macrophages(m?)areprivilegedhostcellsforintracellularbacteriaandthusrepresentalargereservoirofantigenicmaterial,butdcsaremuchmorepotentapc.therefore,intcellimmunitytobacteriatheroleofthesetwophagocyticcellsisnotclearlyestablished.here,weinvestigatedivivotherelativecontributionofm?anddcsuetstotheantibacterialtcellreoetomycobacteriumbovisbcg.twelvehoursafteri.v.administrationofrbcg·male,therateofinfectionintheleenwascomparableform?anddc.